A549 cells (1 × 104 cells per well) were plated onto 96-well plates with 100 µL DMEM/F12 medium and cultured for 12 h in the CO2 incubator. Download GameLoop and use Android emulator to help you play Pokémon UNITE on your laptop or computer.About Pokémon UNIT, The following steps will teach you how to download and play Roblox on PC. Google Scholar, D. Zhi, T. Yang, J. O’Hagan, S. Zhang, R.F. Discover Chicken burrito places near you then order for takeout or pickup online. In vivo NIR-II FI was performed by the small animal NIR-II biological imaging system. Compared with IALP-C4 and RIALP-C4, TIALP-C4 and RRIALP-C4 showed more damage to A549 cells under irradiation. Empaquetadores O Packers De Delivery - Pardos Chicken | [C880] Requisitos: - Experiencia mínima de 06 meses como empaquetador de productos de delivery (en restaurantes). k, l Hydrodynamic size and zeta potentials of IALP-C4, RIALP-C4, TIALP-C4, and RRIALP-C4. Fan, M. Ji, Thiadiazoloquinoxaline derivative-based NIR-II organic molecules for NIR-II fluorescence imaging and photothermal therapy. The results of cell death mode in these two ways correspond to their mechanism. 461. (b) The ratio of IR-1061 to DPPG is 1:20. 8, 12 (2021), Z. A549 cells (1.0 × 105 cells per dish) were plated onto confocal dishes and stained by 500 µL 1 mg/mL Hoechst solution and 200 µL 16 µg/mL DALPs according to the above staining conditions after incubation for 12 h in a CO2 incubator. 7a, the fluorescence intensity of tumor tissue was lighted as soon as 3 h after injection of RRIALP-C4, then reached its maximum at 12 h and was able to maintain for 24 h. Compared with other IALP-C4 liposomes (Additional file 1: Fig. Photothermal therapy (PTT) has been considered a benign therapy for cancer, which has the advantages of low trauma, significant therapeutic effects, and small side … The free drugs group exist the highest cell lethality (60%), which was nearly the sum of single drugs. Small 18, 2202078 (2022), Y. Su, B. Yu, S. Wang, H. Cong, Y. Shen, NIR-II bioimaging of small organic molecule. Descárgalo en la biblioteca de GameLoop o en los resultados de búsqueda. Abra GameLoop y busque "المطبخ العربي - بدون انترنت", busque المطبخ العربي - بدون انترنت en los resultados de búsqueda y haga clic en "Instalar". Practicante de Logísitica (Gestión de Almacenes), Auxiliar de Almacén de Productos Terminados, Ingeniero junior de diseño instrumentista, Jefe de operaciones de atención al cliente, Inicia sesión para crear una alerta de empleo. Delivery Reviews. PoketPOS Digital Menu with Ordering allows customers to view the menu digitally by scanning the displayed QR code. Colloid Surf. Green channel: liposomes. FigureS2. And the Restrained Electrostatic Potential (RESP) charge was calculated by Multiwfn. Empaquetar los pedidos de delivery que lleguen al restaurante. Fan, P. Pei, C. Sun, L. Lu, F. Zhang, Tm3+-sensitized NIR-II fluorescent nanocrystals for in vivo information storage and decoding. Incluye 2 ajíes, 1 mayonesa, 1 vinagreta. The pictures in Fig. Somos Pardos Chicken cadena peruana de restaurantes, líder en el mercado. 3, 8953–8961 (2020), Y. Reconocida por Computrabajo Best Work Place 2020 en el puesto tres entre las mejores empresas para trabajar en Perú, categoría Hoteles y Restaurantes. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. (b) ICG-loadedliposomes (2% molar content) with different phospholipidconcentrations. In addition, for photothermal treatment groups, A549 cells were irradiated from the top of the plate with an 808 nm laser (0.3 W/cm2) for additional 6 min after the addition of liposomes. The results of RIALP-4 and TIALP-4 were consistent with RRIALP-4, indicating that the modification of different types of peptides had no obvious effect on the toxicity of IALPs. Moreover, the tissue penetration depth of the photothermal effect of liposomes was evaluated using chicken tissue in vitro. Galectin-8 binds specific beta1 integrins and induces polarized spreading highlighted by asymmetric lamellipodia in … 5e). a Operation procedure of systems with different number ratios of IR-1061 and phospholipid. Delivery ... Paneer Parda. The developer, Bistrap, indicated that the app’s privacy practices may include handling of data as described below. Privacy Ha ocurrido un error momentáneo. Descargando la aplicación de Cafeladería 4D podrás:Realizar pedidos a tu tiendas más cercana sin necesidad de llamar o escribir por teléfono.Realiza tus pedidos para Delivery o Recojo en Tienda.Acceder a promociones exclusivas para el APP.Conocer la variedad de nuestra carta.Pagar tus pedidos con cualquier medio de pago.Guardar tus direcciones frecuentes.Geolocalización: Encuentra tu dirección rápidamente y haz seguimiento a tus pedidos. III: TIALP-C4. In this work, we report a skillfully fluorescent lipid nanosystem, the RR9 (RGDRRRRRRRRRC) peptide-coated anionic liposome loaded with organic NIR-II fluorophore IR-1061 and chemotherapeutic drug carboplatin, which is named RRIALP-C4. DA and DB means the concentration of combinational drug when cytostatic rate is X. ICXA and ICXB means the concentration of single drug when cytostatic rate is X. Incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta, Un palo de anticucho, no incluye choclo ni papa. Ahora, puedes jugar PoketPOS Digital Menu en PC con GameLoop sin problemas. Download GameLoop and use Android emulator to help you play Roblox on your laptop or computer.About RobloxRoblox is the ultima, The following steps will teach you how to download and play Meta Business Suite on PC. H&E staining sections of organs from differentgroups. b Molecular structure of IR-1061 and DPPG. Nivel mínimo requerido: Secundaria completa. The irradiation of 808 nm laser showed slight damage on A549 cells (86.4% ± 0.91%), but much lower than that co-operated with RRIALP-4 (65.2% ± 0.33%). Servicio en Food Court (Consultar). Coordinate the entry of orders and deliveries of … Se … Incluye 1 ají, 1 mayonesa, 1 vinagreta (No incluye papas), Pardos Parrillero hot con papas fritas y guarnición a elección, 1/2 Pardos Parrillero Hot Solo con Ensalada, 1/2 Pardos Parrillero con papas fritas, guarnición de ensalada a elección y palo de anticucho. Puedes guardar tu currículum y solicitar empleos en cuestión de minutos en LinkedIn. 2c, d. The mass density distribution of systems showed that the total thickness of the liposome bilayer was about 6 nm, and the thickness of the hydrophobic layer ranged from 3 to 3.5 nm. (c) In vivo PTI of TIALP-C4 at different timepoints (0, 15, 30, 60, 90, 120, 180, 240 s). 60, 16294–16308 (2021), Y. Lettuce wilted, chicken dry. Delivery, Ofertas, Promociones solo en Pardos Chicken disfruta el mejor pollo a la brasa. Moreover, the different concentrations of RRIALP-C4 were also evaluated (Additional file 1: Fig. Cell staining was performed by the instructions of the kit and detected by flow cytometry (Thermo Scientific, USA). 5f, i). Se respondió el 7 noviembre 2022 - Delivery - Santiago de Surco, Lima. 1/4 Pollo a la brasa con papas fritas y guarnición de la casa a elección. Chem. they have a menu del dia. Apoyar en las funciones que se le asigne. f H&E and Tunel staining of tumor sections after 15 days of treatment. Conte, S.B. Beneficios corporativos para ti y tu familia. Food is good for the price you pay. In this work, we further visualized the interaction between phospholipid bilayer and NIR-II fluorophore IR-1061 through molecular dynamics simulation to clarify the way in which the H-aggregated state of IR-1061 formed in anionic liposomes (ALPs), and then proposed a method that may enable this aggregated state to be transmitted inside tumor cells to achieve stable and efficient PTT in vivo. The cellular uptake of liposomes was visualized by fluorescence staining. In vitro, photothermal images of RRIALP-C4 were captured by the thermal imager (Ti32, Fluke, US). Sun, J. Ren, T. Jiang, Q. Peng, Q. Ou, Z. Shuai, Intermolecular charge-transfer-induced strong optical emission from herringbone H-aggregates. Soc. Comprehensive and in-depth research on the H-aggregated state of IR-1061 in ALPs was conducted, from theoretical mechanism to intracellular changes. Postular Guardar en Mis favoritos Denunciar empleo Motorizado para Reparto … The fluorescence intensity of liposomes was then detected by the small animal NIR-II biological imaging system under the same condition above. Mol. (a) The linearfitting of − lnθ and time in the cooling curve andphotothermal conversion efficiency. Pardos Chicken ‏يناير 2017 - ‏نوفمبر 2017 11 شهرا. incluye salsas: 2 ajíes, 2 mayonesas, 1 vinagreta. 4, 724–732 (2022), N. Shao, Y. Qi, H. Lu, D. He, B. Li, Y. Huang, Photostability highly improved nanoparticles based on IR-780 and negative charged copolymer for enhanced photothermal therapy. Rev. This is the version of our website addressed to speakers of English in India. Incluye salsas: 2 ajíes, 1 mayonesa, 1 vinagreta (No incluye papas), 1/2 Parrillero original con papas fritas, guarnición de ensalada a elección y palo de anticucho. In addition, the uptake mechanism of ALPs and the PTT effect after uptaken by A549 cells were also evaluated to investigate whether the intraliposomal H-aggregated state of IR-1061 could be maintained inside tumor cells. S18). Liu, X.H. Incluye salsas: 2 ajíes, 2 mayonesa, 2 vinagretas, 1 pollo a la brasa, papas, complemento y bebida a elección. Terms and Conditions, Fluorescence images of A549cells phagocytosis of liposomes. For this reason, RhB-ALPs (RALPs) and DiO-ALPs (DALPs) were prepared separately and collocated their confocal fluorescence signals with DRALPs to evaluate the uptake mechanism of ALPs. Discover Chicken burrito places near you then order for takeout or pickup online. Figure S10. The evaluation criteria of IR-1061 in the H-aggregated state is that 2 molecules are arranged face-to-face with a distance less than 5 Å. (a) ICG aqueoussolution with different concentrations. Haz clic en "Continuar" para acceder a tu cuenta o crear una cuenta nueva. We believe this mechanism is universal, that is, using the characteristics of liposomes to modify the NIR-II fluorophore to make it have a variety of aggregated states is a new research direction, and has great application prospects. 141, 19221–19225 (2019), C. Sun, M. Zhao, X. Zhu, P. Pei, F. Zhang, One-Pot preparation of highly dispersed second near-infrared J-aggregate nanoparticles based on FD-1080 cyanine dye for bioimaging and biosensing. By using this website, you agree to our Ambala Locality, Ambala. Characterization of liposomes. The mean hydrodynamic size and ζ-potential of liposomes were measured by a Malvern Zeta-sizer Nano instrument (Malvern Instruments Ltd, Malvern, UK). J. Photochem. Puedes actualizar tus preferencias en cualquier momento en tus ajustes. (b) In vivo NIR-II FI of TIALP-C4 at differenttime points (0, 1, 3, 6, 12, 24 h). The combinational index of Carbo + IALP-4, ALP-C + IALP-4, and IALP-C4 were calculated respectively. The A549 cells incubated with RRIALP-4 at different times, and then detected their temperature rise curves with an infrared thermal imager. ¿Cuál es la calificación que nuestros clientes le dan a … While RRIALP-C4 possessed the highest degree of enrichment in tumor tissue, almost the same as liver, indicating that RRIALP-C4 had a satisfactory tumor-targeting ability. View Summer Pardo, PWS, CWB®’s profile on LinkedIn, the world’s largest professional community. In addition, the fluorescence images of normal organs (heart, liver, spleen, lung, and kidneys) and tumors of mice treated with RRIALP-C4 were harvested to evaluate its distribution in vivo (Fig. … Therefore, a rational design of a nanosystem can not only improve the biocompatibility of fluorophores but also possess an ideal spatial structure to change their aggregated state. After that, the residual organic solvent in the reaction solution was removed by rotary evaporation and the original liposome solution was supplemented to a phospholipid concentration of 10 mg/mL. ACS Biomater. S8). As ALPs were engulfed by A549 cells in the form of membrane fusion, IR-1061 was able to be transferred from liposomes to the cell membrane only, thus further maintaining its H-aggregated state. Similarly, the preparation process of IR1061-ALP (IALP) is identical to that of IALP-C except that carboplatin was not added. B 156, 38–43 (2017), J.A. Juguemos المطبخ العربي - بدون انترنت y … While in the RRDALP group, the vast majority of DiO showed a distribution around the cells (Fig. The characteristics of the blue shift peak are completely consistent with that of the fluorophore under the H-aggregated state, meaning the increase of IR-1061 content in IALPs will increase its proportion in the H-aggregated state and reduce that of the free state [30]. The photothermal properties of liposome solution were measured by optical fiber thermometry. Nuestra Carta Salón. This review is the subjective opinion of a Tripadvisor member and not of Tripadvisor LLC. All in vivo procedures were performed under a protocol approved by the Institutional Animal Care and Use Committee of Southeast University. There was no significant cytotoxicity when blank RRALP (cell viability: 97.5% ± 1.8%) and RRIALP-4 (95.3 ± 2.6%) interacted with A549 cells, while the carboplatin-loaded RRALPs possessed certain cytotoxicity (RRALP-C: 73.5% ± 1.4%, RRIALP-C4: 72.1% ± 3.2%). (c) Maximum emission peak of DiO-ALP. Te invitamos a vivir esta gran experiencia compartiendo siempre FELICIDAD! Xu, N. Gu, Y. Zhang, Fe3O4@Pt nanozymes combining with CXCR4 antagonists to synergistically treat acute myeloid leukemia. However, limited by the competitive relationship between radiative and nonradiative transitions, small organic molecular fluorophores often exhibit excellent fluorescence properties but lack efficient photothermal conversion efficiency [12, 13]. Dining options: After-hours, Delivery, Reservations . g–j TEM images of IALP-C4, RIALP-C4, TIALP-C4 and RRIALP-C4. Transmissible H-aggregated NIR-II fluorophore to the tumor cell membrane for enhanced PTT and synergistic therapy of cancer, $${\upeta}=\frac{\text{hS}({\text{T}}_{\text{max}}-{\text{T}}_{\text{suur}})-{\text{Q}}_{\text{dis}}}{\text{I}(1-{10}^{-{\text{A}}_{808}})}$$, $${{\uptau }}_{\text{s}}=\frac{{m}_{D}{C}_{D}}{hS}$$, \(\text{CI}=\frac{{D}_{A}}{{ICX}_{A}}+\frac{{D}_{B}}{{ICX}_{B}}\), https://doi.org/10.1186/s40580-022-00352-4, http://creativecommons.org/licenses/by/4.0/. Nat. HORRIBLE service HORRIBLE place and BAD FOOD. المطبخ العربي - بدون انترنت para PC en el emulador de Android le permitirá tener una experiencia móvil más emocionante en una computadora con Windows. 1b, c). Cell staining was performed with the instructions of the kit. Servicio de Salón. Absorption peaks of purified cell membrane after Incubated with IALP-4. RR9 peptide is synthesized from a stretch of positively charged arginine chains with RGD peptide, which integrates the functions of both peptides [29]. So we waited an hour. The mixed solution was added dropwise to deionized water dissolved with 1 mg carboplatin and stirred continuously under 50 °C for 30 min. The modification of liposome surface peptide only changed the amount of RhB in cells but did not change its distribution (Additional file 1: Fig. المطبخ العربي - بدون انترنت para PC en el emulador de Android le permitirá tener una experiencia móvil más emocionante en una computadora con Windows. It can be used with any mobile device that has an option to scan the QR code. Cell staining was observed by a laser confocal microscope. We order two plates. The parameters S, h, Tmax, Tsurr, Qdis, I and A808 represent the container’s surface area, heat-transfer coefficient, maximum laser-trigger temperature, indoor temperature, heat dissipation caused by the light-absorbing of quartz cuvette, the intensity of laser (0.3 W/cm2) and absorbance of liposomes at 808 nm. Areas de Juegos para Niños… Then 0.2 mL liposome solution (2.5 mg/mL) was injected intravenously before imaging. The single liposomes combi group showed a cell lethality (42%), which was lower than IALP-C4 and free drugs group. Photobiol. 4a, RRIALP-4 had little significant cytotoxicity (> 95%) to A549 cells at a phospholipid concentration lower than 2 mg/mL, which contained the appropriate concentration for tumor PTT. Te recomendamos que lo completes para que el empleador pueda conocerte. The operation process was shown in Fig. Donnelly, Photothermal therapy. incluye salsas: 1 ají, 1 vinagreta (no incluye papas). These results proved that IALPs were absorbed by A549 cells in the form of membrane fusion, and the distribution of IR-1061 would be dispersed on the cell membrane, similar with DiO. The fluorescence and photothermal properties of RRIALP-C4 presented a dual-channel activated integration of intravital NIR-II FI under the excitation of 1061 nm and NIR-I PTT excited by 808 nm. S3e, S4b). f Thermal images of RRIALP-C4 (10 mg/mL) after being irradiated with 808 nm laser at 0.3 W/cm2 in 24-well plates (control: cell culture medium). By integrating RGD peptide function and TAT peptide function, RR9 modified DRALP (RRDRALP) had the maximum fluorescence intensity. Ingreso a planilla desde el primer día con los beneficios de ley. Debes iniciar sesión en tu cuenta para agregar este empleo a tus favoritos. Incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta (No incluye papas), 1/2 Parrillero Original con Papas y Ensalada, 1/2 Parrillero original (pierna y pecho) con papas fritas y guarnición a elección. Almuerzo laboral, SCTR, Campaña escolar, premiación al trabajador del mes, descuento por consumos, Seguro Vida Ley, Capacitación constante, Oportunidad de Línea de Carrera, Beneficios corporativos para ti y tu familia entre otros. Thermalimages of A549 cells treated with RRIALP-4 (2 mg/mL) in different times (irradiatedwith 808 nm laser at 0.3 W/cm2 for 5 min). Blue channel: nucleus. Cookies policy. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Furthermore, the fluorescence signal intensity of the abdominal and hind limb blood vessels of mice were analyzed (Fig. All these results demonstrated that RRIALP-C4 could achieve a great tumor accumulation and efficiently suppress tumor growth without any appreciable systemic toxicity. CI means combinational index. Int. Preguntada 7 noviembre 2022. Next, liposomes modified with three types of peptides (RGD, TAT48–60, and RR9) and encapsulated with carboplatin were named RIALP-C4, TIALP-C4, and RRIALP-C4, were for subsequent studies. Int. Askari Rizvi, H. Zhang, Emerging trends of receptor-mediated tumor targeting peptides: a review with perspective from molecular imaging modalities. Consulta más información en nuestra Política de cookies. Bioactive Mater. Chem. The loading efficiency and release rate of drugs in liposomes were calculated by the content of residual drugs in the filtrate. Fang Yang, Peng Wang or Min Ji. Cell viability was detected using MTT assays. In vivo synergistic therapy of RR-ICG-ALP andsingle liosome combi group (RRALP-C+RRIALP-4+L). Q1, Q2, Q3, and Q4 represent necrotic, lateapoptotic, early apoptotic, and viable cells, respectively. 21, 5394–5400 (2021), K. Li, X. Duan, Z. Jiang, D. Ding, Y. Chen, G. Zhang, Z. Liu, J-Aggregates of meso-[2.2]paracyclophanyl-BODIPY dye for NIR-II imaging. The photostability of RRIALP-C4 was also examined by cyclic lift temperature (Fig. Somos Pardos Chicken cadena peruana de restaurantes, líder en el mercado. Cell targeting and uptakes of liposomes. Teletrabajo Moreover, to prove the excellent photothermal performance of RRIALP-C4, we simultaneously prepared ICG-encapsulated ALP (ICG-ALP) and IR-780-loaded ALP (IR780-ALP), and tested their photothermal stability (Additional file 1: Figs. The results showed extremely low leakage of IR-1061 and unreduced H-aggregated form under short-term temperature circulations. Google Scholar, Y. Yang, C. Sun, S. Wang, K. Yan, M. Zhao, B. Wu, F. Zhang, Counterion-paired bright heptamethine fluorophores with NIR-II excitation and emission enable multiplexed biomedical imaging. Farokhzad, Theranostic nanomedicine in the NIR-II window: classification, fabrication, and biomedical applications. S2). (a) A549 cells treated with DRALP, RRDRALP,and RRDRALP+Pitstop 2 for 30 min. Water geometry was constrained using the SETTLE algorithm. IR-780 was also reported to have good photothermal properties [32]. These results were also demonstrated in Fig. incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta. Sci. With this license,items can be added to the order, customizations done as per the preference of the customer, and integration of all modes of payments is possible. (f) Maximum emissionpeak of Rhb-ALP. d Images of tumor changes with the synergistic treatment of RRIAP-C4 for 15 days. Figure S5. Dining Reviews. Incluye salsas: 2 ajíes, 1 mayonesa, 1 vinagreta, 1/2 Parrillero Original con Solo Ensalada, 1/2 Parrillero original con ensalada. ICG is a clinical contrast agent and has been proved to possess photothermal properties. Biomaterials 271, 120717 (2021), S. Gao, G. Wei, S. Zhang, B. Zheng, J. Xu, G. Chen, M. Li, S. Song, W. Fu, Z. Xiao, Albumin tailoring fluorescence and photothermal conversion effect of near-infrared-II fluorophore with aggregation-induced emission characteristics. View Summer Pardo, PWS, CWB®’s profile on LinkedIn, the world’s largest professional community. (b) In vivo PTI of RIALP-C4 at different time points (0,15, 30, 60, 90, 120, 180, 240 s). S5). To investigate the occurrence of the H-aggregated state of IR-1061 in ALPs and its mechanism, a total of 5 kinds of ALPs, named IALP-1 (2, 3, 4, 5), with different molar contents (compared with phospholipid) of IR-1061 (0.5, 1, 1.5, 2, and 2.5%) were synthesized and their optical spectral properties were analyzed. This indicated that this group eventually recruited less IR-1061 and carboplatin in the tumor tissue, illustrating the emergence of a competitive relationship between these two liposomes. Inter. This manuscript was supported by the National Natural Science Foundation of China (Grant Numbers 81671745, 82071979) and the Suzhou key industry technology innovation project (Grant Number SYG201912). Ed. Zhang, B.Y. The 1064 OD filter can cut off the wavelength at 1064 nm and avoid the background interference of the excitation wavelength. S13). The emitted fluorescence was captured by a NIR-II camera with a 1064 nm long-pass filter (1064 LP) and a 1064 nm cut-off filter (1064 OD) under an exposure time of 300 ms. To further prove the way of liposomes entering A549 cells, we used the endocytosis inhibitor, Pitstop 2, to inhibit the clathrin mediated cytocytosis (Additional file 1: Fig. 1d). 2. TIALP-C4 and RRIALP-C4 showed almost no size changes within 3 weeks and slight size changes within 1 month, indicating the positive charge modifications of IALP-C4 also provided excellent stability. Haz clic en el email que te hemos enviado a para verificar tu dirección y activar la alerta de empleo. As shown in Fig. Specifically, after incubating A549 cells with IALP-4 for different time, we removed free liposomes by centrifugation, then cells were split using cell lysate and freeze-thaw method. Estos son los campos que te faltan por llenar: Este error puede que haya sucedido por dos motivos:1. We have achieved the coexistence of the H-aggregated state of IR-1061 with the free state through the unique phospholipid bilayer structure of liposomes, which cannot be achieved in other solvent environments. Tumor-bearing mice were developed by subcutaneous transplantation of A549 cells (1 × 107 cells/mice) on mice (20 ± 2 g, 5–6 weeks). - … Mice treated with RRIALP-4 alone showed no treatment effect compared with that treated with PBS. The synergistic therapy of RRIALPs (1 mg/mL) to A549 cells was detected by cell viability detection and cell apoptosis detection. 4b). Control Release 325, 52–71 (2020), Q. Cheng, T. Li, Y. Tian, H. Dang, H. Qian, C. Teng, K. Xie, L. Yan, NIR-II fluorescence imaging-guided photothermal therapy with amphiphilic polypeptide nanoparticles encapsulating organic NIR-II dye. Lee, D. Wang, B.Z. Requisitos Thermal images of A549 cells treated with ICG aqueoussolution and ICG-ALP in different times. Servicio de Delivery. (a) Cytotoxicity of A549 cells treated with drugs group (6.7 μM Carbo+1mg/mL IALP-4+L) and single liposomes combi group (1mg/mL ALP-C+1mg/mL IALP+L) aftertreatment for 24 h. (b) Flow cytometry of apoptosis cells treated withdifferent conditions.
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